Effect of pretreatment of soy insoluble fiber and SSCF with Saccharomyces cerevisiae and Escherichia coli KO11 on ethanol production in an integrated corn-soy biorefinery
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Since 1905, the Department of Agricultural Engineering, now the Department of Agricultural and Biosystems Engineering (ABE), has been a leader in providing engineering solutions to agricultural problems in the United States and the world. The department’s original mission was to mechanize agriculture. That mission has evolved to encompass a global view of the entire food production system–the wise management of natural resources in the production, processing, storage, handling, and use of food fiber and other biological products.
History
In 1905 Agricultural Engineering was recognized as a subdivision of the Department of Agronomy, and in 1907 it was recognized as a unique department. It was renamed the Department of Agricultural and Biosystems Engineering in 1990. The department merged with the Department of Industrial Education and Technology in 2004.
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1905–present
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- Department of Agricultural Engineering (1907–1990)
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- College of Agriculture and Life Sciences (parent college)
- College of Engineering (parent college)
- Department of Industrial Education and Technology, (merged, 2004)
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Abstract
Soy insoluble fiber (IF), co-product of enzyme-assisted aqueous extraction process (EAEP) of soybeans, is rich in carbohydrate and protein. It can be used to enhance ethanol production in an integrated corn-soy biorefinery, which integrates components from soybean processing into corn-based ethanol processing. However, cornstarch and IF have unique carbohydrate compositions that require different treatments for optimal fermentation. The present study investigated the effect of pretreatment method [soaking in aqueous ammonia (SAA), liquid hot water (LHW), and enzymatic hydrolysis], simultaneous saccharification and co-fermentation (SSCF) with Saccharomyces cerevisiae and Escherichia coli KO11, and scaling up from bench scale (150 mL) to pilot scale (60 L) on ethanol production in IF fermentation. Untreated IF was added to integrated corn-soy fermentation and the effect of SSCF was evaluated. It was demonstrated that enzymatic hydrolysis with enzyme cocktail of pectinase, cellulase and xylanase, is the best pretreatment method to maximize ethanol production in IF fermentation with an added advantage of adding enzymes to the fermentation slurry at the SSF step. Ethanol yield almost doubled when SSCF of IF was performed with E. coli KO11 due to conversion of arabinose and xylose into ethanol. Addition of untreated IF to dry-grind corn fermentation increased ethanol production rate, but low ethanol tolerance of E. coli KO11 was a limiting factor in achieving SSCF with S. cerevisiae and E. coli KO11. Michaelis-Menten equation accurately predicted E. coli KO11 growth kinetics by Hanes-Woolf linearization.
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This paper is from 2015 ASABE Annual International Meeting, Paper No. 152190086, pages 1-14 (doi: 10.13031/aim.20152190086). St. Joseph, Mich.: ASABE. Posted with permission.