Agricultural and Biosystems Engineering Publications

Document Type

Article

Publication Date

2007

Journal or Book Title

Veterinary Research

Volume

38

Issue

12

First Page

81

Last Page

93

Research Focus Area(s)

Animal Production Systems Engineering

DOI

10.1051/vetres:2006044

Abstract

The objective of this experiment was to describe the stability of airborne infectious porcine reproductive and respiratory syndrome virus (PRRSV) as a function of temperature and relative humidity. A cloud of infectious PRRSV was aerosolized using 24-jet Collison nebulizer into a dynamic aerosoltoroid (DAT) maintained at a specific temperature and relative humidity. The PRRSV cloud within the DAT was sampled repeatedly over time using SKC BioSampler impingers and the total viral RNA (RT-PCR) and concentration of infectious PRRSV (TCID in the air samples was determined. As measured by quantitative RT-PCR, PRRSV RNA was stable under the conditions evaluated in this study. Thus, a comparison of viral RNA and Rhodamine B dye, a physical tracer, found no significant difference in the slopes of the lines. Titers of infectious virus were plotted by time and the half-life (1/2) of infectious PRRSV was calculated using linear regression analysis. An analysis of the results showed that aerosolized PRRSV was more stable at lower temperatures and/or lower relative humidity, but temperature had a greater effect on the 1/2 of PRRSV than relative humidity. Based on these results, an equation was derived to predict the 1/2 of infectious airborne PRRSV for any combination of environmental temperature and relative humidity.

Comments

This article is from Veterinary Research 38, no. 1 (2007): 81–93, doi:10.1051/vetres:2006044. Posted with permission.

Copyright Owner

INRA, EDP Sciences

Language

en

Date Available

May 1, 2013

File Format

application/pdf

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