Agricultural and Biosystems Engineering Publications

Document Type

Article

Publication Date

9-13-2013

Journal or Book Title

Biosensors

Volume

3

Issue

3

First Page

312

Last Page

326

Research Focus Area(s)

Biological and Process Engineering and Technology

DOI

10.3390/bios3030312

Abstract

Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

Comments

This article is from Biosensors 3 (2013): 312–326, doi:10.3390/bios3030312.

Rights

© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

Copyright Owner

The authors

Language

en

Date Available

2013-10-31

File Format

application/pdf

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