Reduction of ammonia emissions from animal feeding operations is important from the perspective of environmental policy and its impact on agriculture. A laboratory study was conducted to evaluate how rate and frequency of urease inhibitor application affect ammonia emissions from simulated beef cattle feedyard manure surfaces. The urease inhibitor N-(n-butyl)thiophosphoric triamide (NBPT) was applied at rates of 0, 1, and 2 kg ha-1, at 8, 16, and 32 day frequencies, and with or without simulated rainfall. Synthetic urine was added every two days to the manure surface. Gaseous ammonia was trapped by bubbling through a sulfuric acid solution using a vacuum system and analyzed for nitrogen using automated procedures. NBPT applied every 8 days was most effective, with the 1 and 2 kg NBPT ha-1 treatments resulting in 49% to 69% reduction in ammonia emission rates, respectively. The 8-day, 1 kg NBPT ha-1 treatments had the most promising benefit/cost ratios of 0.48 to 0.60. Simulated rainfall reduced the ammonia emission rates from 1% to 25% as compared to the non-rainfall treatments, although the differences were not statistically different. The use of NBPT for reducing ammonia emissions looks promising; however, possible buildup of urea in the pen surface may require a higher NBPT application rate with time.