Summary and Implications
Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with Xba1. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using Xba1 restriction provides a screening method for identifying swine Salmonella serotypes.
Iowa State University
Gaul, Stephen B.; Wedel, Stephanie; Erdman, Matthew M.; Harris, D.L. Hank; Harris, Isabel Turney; Ferris, Kathleen E.; and Hoffman, Lorraine J.
"Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments,"
Animal Industry Report:
AS 653, ASL R2176.
Available at: http://lib.dr.iastate.edu/ans_air/vol653/iss1/3