Summary and Implications
The immune responses of 390 BALB/c mice fed the probiotic Lactobacillus acidophilus strain NP51 ® and infected with Mycobacterium avium subspecies paratuberculosis (MAP) were evaluated in a 6-month trial. Mice were randomized to nine treatment groups that fed either viable- or heat-killed NP51 and inoculated with either viable- or heatkilled MAP or sterile phosphate-buffered saline. Feeding the NP51 resulted in higher numbers of T lymphocytes in the spleen including the CD8 + cytotoxic T lymphocytes. In addition, feeding the NP51 lowered the number of immune suppressive T regulatory cells CD4 + CD25 + and CD8 + CD25 + cells in the spleen. Additionally, feeding the NP51 resulted in higher concentration of interferon-gamma in the supernatant of splenocytes cultured in vitro. These results suggest that feeding the NP51 to BALB/c mice might prevent the progression of MAP infection in mice.
Iowa State University
Osman, Mohamed; Stabel, Judith; Hostetter, Jesse M.; Nettleton, Daniel S.; and Beitz, Donald C.
"Prevention of Mycobacterium avium Subspecies paratuberculosis
(MAP) Infection in Balb/c mice by Feeding Lactobacillus
acidophilus Strain NP-51®,"
Animal Industry Report:
AS 656, ASL R2487.
Available at: http://lib.dr.iastate.edu/ans_air/vol656/iss1/8