Lipopolysaccharide (LPS) is a component of gram-negative bacterial cell walls that can stimulate a cellular inflammatory reaction. The objective of this study was to determine the effect of genetic line and time on LPS-induced pro-inflammatory response. Thirty roosters were divided into two groups of challenge and non-challenge for this experiment. The challenged birds were intravenously injected with LPS, to characterize the genetic response to inflammatory stimuli. At hours one and three post-injection, blood was collected, white blood cells (WBC) were isolated, and RNA isolated from the WBC. Real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to estimate gene expression levels from mRNA. In summary, all three genetic lines responded to LPS with enhanced gene expression, and the three unique genetic lines differed both in baseline levels and in LPS response in mRNA expression of IL-6 and IFN-γ cytokines. The results indicate that there is a genetic component associated with inflammation response.