The RNA expression levels of Caspase 1 and Interleukin 18 were quantified after a lipopolysaccharide (LPS) challenge. The proteins of these two genes are specifically involved with the inflammatory immunological response pathway of the chicken. Collectively, a greater understanding of RNA expression levels of immunologically related genes in the chicken genome under challenging situations could lead to increased effectiveness of future genetic selection and targeted therapeutics against prevalent diseases in the poultry industry. Results demonstrated the effectiveness of the LPS stimulation, and variation in expression among genetic lines.