Rapid Communication: Mapping the Pig VCAM1 Locus to Chromosome 4 Using a Double-Stranded Conformation Polymorphism Marker (VCAM1-2)
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1997
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Rothschild, Max
Distinguished Professor Emeritus
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Animal Science
Abstract
Source and Description of Primers. We previously identified a SacI polymorphism by using a pig VCAM1 cDNA probe on Southern blots (VCAM1-1; Helm et al., 1994). This polymorphism was not informative enough to map VCAM1. To develop PCR-based genotyping, we sequenced the 3¢ untranslated region of pig VCAM1. Subsequently, a pig VCAM1 cDNA was deposited in Genbank; our data agree completely with that reported by Tsang et al. (Accession: U08351). The PCR primers were designed (forward, 5¢-TATCAGCCCTCCATAGTCACAT 3¢ and reverse, 5¢- GAAATTGTTGTCCATGACCTTTAT 3¢) .
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This is an article from Journal of Animal Science 75 (1997): 2286, doi:/1997.7582286x. Posted with permission.
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Wed Jan 01 00:00:00 UTC 1997