Campus Units

Biochemistry, Biophysics and Molecular Biology, Ames Laboratory

Document Type

Conference Proceeding

Conference

Medical Imaging 2015: Digital Pathology

Publication Version

Published Version

Publication Date

2015

Journal or Book Title

Proceedings of SPIE

Volume

9420

First Page

1

Last Page

4

DOI

10.1117/12.2081691

Conference Title

Medical Imaging 2015: Digital Pathology

Conference Date

February 21, 2015

City

Orlando, FL

Abstract

In this work we present a time-lapsed confocal microscopy image analysis technique for an automated gene expression study of multiple single living cells. Fluorescence Resonance Energy Transfer (FRET) is a technology by which molecule-to-molecule interactions are visualized. We analyzed a dynamic series of ~102 images obtained using confocal microscopy of fluorescence in yeast cells containing RNA reporters that give a FRET signal when the gene promoter is activated. For each time frame, separate images are available for three spectral channels and the integrated intensity snapshot of the system. A large number of time-lapsed frames must be analyzed to identify each cell individually across time and space, as it is moving in and out of the focal plane of the microscope. This makes it a difficult image processing problem. We have proposed an algorithm here, based on scale-space technique, which solves the problem satisfactorily. The algorithm has multiple directions for even further improvement. The ability to rapidly measure changes in gene expression simultaneously in many cells in a population will open the opportunity for real-time studies of the heterogeneity of genetic response in a living cell population and the interactions between cells that occur in a mixed population, such as the ones found in the organs and tissues of multicellular organisms.

Comments

This is a proceeding from Medical Imaging 2015: Digital Pathology 9420 (2015): 1, doi:10.1117/12.2081691. Posted with permission.

Copyright Owner

SPIE

Language

en

File Format

application/pdf

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