Document Type

Article

Publication Date

5-2007

Journal or Book Title

Journal of the American Chemical Society

Volume

129

Issue

21

First Page

6684

Last Page

6685

DOI

10.1021/ja071158n

Abstract

Labdane-related diterpenoids are a large group of over 5,000 natural products whose biosynthesis typically proceeds through a labdadienyl/copalyl diphosphate (CPP) intermediate to a further cyclized and/or rearranged hydrocarbon diterpene en route to more elaborated compounds. Here we report a modular approach for facile biosynthesis of labdane-related diterpenes wherein base pGGxC vectors capable of introducing bacterial production of any one of the three common stereoisomers of CPP can be co-introduced with diterpene synthases that convert these CPP intermediates to specific diterpene hydrocarbon skeletal structures. The utility of this approach is demonstrated by individually engineering E. coli to produce any one of eight different diterpene skeletal structures, which collectively serve as precursors to literally thousands of distinct natural products.

Comments

Reprinted with permission from Journal of the American Chemical Society 129 (2007): 6684, doi:10.1021/ja071158n. Copyright 2007 American Chemical Society.

Rights

One-time permission is granted only for the use specified in your request. No additional uses are granted (such as derivative works or other editions). For any other uses, please submit a new request.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

2007_Peters_ModularApproachSupplement.pdf (516 kB)
supplemental material

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