Campus Units

Biochemistry, Biophysics and Molecular Biology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

5-1-2011

Journal or Book Title

Biochemical Journal

Volume

435

Issue

3

First Page

589

Last Page

595

DOI

10.1042/BJ20101429

Abstract

The evolution of natural products biosynthetic pathways can be envisioned to occur via a number of mechanisms. Here we provide evidence that latent plasticity plays a role in such metabolic evolution. In particular, rice (Oryza sativa) produces both ent- and syn-copalyl diphosphate (CPP), which are substrates for downstream diterpene synthases. Here we report that several members of this enzymatic family exhibit dual reactivity with some pairing of ent-, syn-, or normal CPP stereochemistry. Evident plasticity was observed, as a previously reported entsandaracopimaradiene synthase also converts syn-CPP to syn-labda-8(17),12E,14-triene, which can be found in planta. Notably, normal CPP is not naturally found in rice. Thus, the presence of diterpene synthases that react with this non-native metabolite reveals latent enzymatic/metabolic plasticity, providing biochemical capacity for utilization of such a novel substrate (i.e., normal CPP) that may arise during evolution, the implications of which are discussed.

Comments

This is a pre-publication manuscript of an article published as Morrone, Dana, Matthew L. Hillwig, Matthew E. Mead, Luke Lowry, D. Bruce Fulton, and Reuben J. Peters. "Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals." Biochemical Journal 435, no. 3 (2011): 589-595. doi: 10.1042/BJ20101429. Posted with permission.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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