Campus Units

Biochemistry, Biophysics and Molecular Biology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

9-11-2015

Journal or Book Title

Chemical Communications

Volume

51

Issue

70

First Page

13485

Last Page

13487

DOI

10.1039/C5CC05754J

Abstract

While cyclic ether forming terpene synthases are known, the basis for such heterocyclisation is unclear. Here it is reported that numerous (di)terpene synthases, particularly including the ancestral ent-kaurene synthase, efficiently produce isomers of manoyl oxide from the stereochemically appropriate substrate. Accordingly, such heterocyclisation is easily accomplished by terpene synthases. Indeed, the use of single residue changes to induce production of the appropriate substrate in the upstream active site leads to efficient bifunctional enzymes producing isomers of manoyl oxide, representing novel enzymatic activity.

Comments

This is a manuscript of an article published as Mafu, S., K. C. Potter, M. L. Hillwig, S. Schulte, J. Criswell, and R. J. Peters. "Efficient heterocyclisation by (di) terpene synthases." Chemical communications 51, no. 70 (2015): 13485-13487. doi: 10.1039/C5CC05754J. Posted with permission.

Copyright Owner

The Royal Society of Chemistry

Language

en

File Format

application/pdf

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