Campus Units

Biochemistry, Biophysics and Molecular Biology, Food Science and Human Nutrition, Center for Crops Utilization Research, Botany

Document Type

Article

Publication Version

Published Version

Publication Date

1990

Journal or Book Title

Nucleic Acids Research

Volume

18

Issue

9

First Page

2826

Last Page

2826

Abstract

We have screened a lambda gtlO cDNA library synthesized from poly A RNA isolated from carrot somatic embryos to identify genes whose expression was associated with embryogenesis. 200,000 recombinant phage were differentially hyridized with single-stranded, 32P-labelled cDNA synthesized from poly A RNA isoalted from callus cells (1) and from embryos (2) of Daucus carota. This screen identified EMB-1, an mRNA that uniquely accumulates in embryos. The derived amino acid sequence of this clone shows similarity to the Em protein of wheat (3) and the D19 mRNA and protein of cotton (4). These proteins are translated from Lea mRNAs, which accumulate to high levels in very late staged zygotic embryos of wheat and cotton, respectively (3, 5). This is the first report of a Lea protein of group 1(4) accumulating in somatic embryos.

Comments

This is an article from Nucleic Acids Research 18 (1990): 2826. Posted with permission.

Rights

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Copyright Owner

Oxford University Press

Language

en

File Format

application/pdf

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