Campus Units

Biochemistry, Biophysics and Molecular Biology

Document Type

Article

Publication Version

Published Version

Publication Date

3-2015

Journal or Book Title

Acta Crystallographica Section F:Structural Biology Communications

Volume

F71

Issue

3

First Page

333

Last Page

337

DOI

10.1107/S2053230X15002423

Abstract

Infection by the parasite Plasmodium falciparum is the leading cause of malaria in humans. The parasite has a unique and essential plastid-like organelle called the apicoplast. The apicoplast contains a genome that undergoes replication and repair through the action of a replicative polymerase (apPOL). apPOL has no direct orthologs in mammalian polymerases and is therefore an attractive antimalarial drug target. No structural information exists for apPOL, and the Klenow fragment of Escherichia coli DNA polymerase I, which is its closest structural homolog, shares only 28% sequence identity. Here, conditions for the crystallization of and preliminary X-ray diffraction data from crystals of P. falciparum apPOL are reported. Data complete to 3.5 Å resolution were collected from a single crystal (2 × 2 × 5 µm) using a 5 µm beam. The space group P6522 (unit-cell parameters a = b = 141.8, c = 149.7 Å, α = β = 90, γ = 120°) was confirmed by molecular replacement. Refinement is in progress.

Comments

This article is from Acta Crystallographica Section F:Structural Biology Communications 71 (2015): 333, doi: 10.1107/S2053230X15002423. Posted with permission.

Copyright Owner

International Union of Crystallography

Language

en

File Format

application/pdf

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