Campus Units

Biochemistry, Biophysics and Molecular Biology

Document Type

Article

Publication Version

Published Version

Publication Date

2016

Journal or Book Title

Organic Letters

Volume

18

Issue

5

First Page

1060

Last Page

1063

DOI

10.1021/acs.orglett.6b00181

Abstract

Through site-directed mutagenesis targeted at identification of the catalytic base in the rice (Oryza sativa) syn-copalyl diphosphate synthase OsCPS4, changes to a single residue (H501) were found to induce rearrangement rather than immediate deprotonation of the initially formed bicycle, leading to production of the novel compound syn-halimadienyl diphosphate. These mutational results are combined with quantum chemical calculations to provide insight into the underlying reaction mechanism.

Comments

This article is published as Product Rearrangement from Altering a Single Residue in the Rice syn-Copalyl Diphosphate Synthase, Kevin C. Potter, Meirong Jia, Young J. Hong, Dean Tantillo, and Reuben J. Peters, Organic Letters 2016 18 (5), 1060-1063, DOI: 10.1021/acs.orglett.6b00181. Posted with permission.

Rights

This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

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