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The Twenty-Seventh Annual Biochemical Engineering Symposium was held at Colorado State University on September 13, 1997. Approximately seventy students and faculty from six universities (Colorado State University, Iowa State University, Kansas State University, University of Colorado, University of Oklahoma, and University of Wyoming) attended the symposium. The program consisted of nine oral presentations and twenty posters. As has been the practice at previous symposia in this series, all presentations represented ongoing or completed projects conducted by graduate students from the participating universities. Fifteen authors submitted full papers for this proceedings, and seven others contributed summary abstracts. Most describe work in progress that will be discussed more fully in subsequent articles appearing in refereed journals.

Contents

Mutations to Alter Aspergillus awamori Glucoamylase Selectivity - Hsuan-Liang Liu, Pedro M. Coutinho, Clark Ford, and Peter J. Reilly

New Fusion Protein Systems Designed to Increase Soluble Cytoplasmic Expression of Heterologous Proteins in Escherichia coli - Gregory D. Davis, Claude Elisee, Denton M. Newham, and Roger G. Harrison

Maximizing Protein Synthesis in High Cell Density Perfusion Bioreactors - Frank W. F. Lee, Stephanie Bryant, Paul Todd, and Dhinakar Kompala

Identification of Different Phases in a Fermentation Using Neural Networks - Laurent Simon, M. Nazmul Karim, and Amanda Schreiweis

Cascade Concentration of Spirulina platensis Based on Inertial Migration - S. R. Dahl, A. L. Rakow, and K. C. Ryan

Cross-Flow Filtration of Bacterial Lysate with Flocculation - Hugh Graham, M. N. Karim, and A. S. Cibulskas

The Effect of Secondary Membranes on Protein Transmission in a Multicomponent System - Vinod Kuberkar and Robert H. Davis

Lyophilization Induced Phase Separation - Effects on Hemoglobin Structure: Martin C. Heller, Theodore W. Randolph, and John F. Carpenter

The Effect of Nonionic Surfactants on the Stability of Lyophilized CNTF - LaToya Jones, John Carpenter, Theodore Randolph, and B.S. Chang

Effect of High Pressure on Crystallization of Subtilisin Mutants - R. Waghmare, M. Joona, M. Larson, C. Glatz, J. Webb, and T. Randolph

Isoelectric Precipitation of Recombinant Proteins from Canota Extracts - Ferhana Zaman, Jocelyn Pollack, and Charles E. Glatz

Recovery of T4 Lysozymes from Canota Protein Extract by Adsorption Chromatography - Chenming Zhang and Charles E. Glatz

Development of a Biosensor for 1,2-Dichloroethane - Derek W. Campbell, Cord Mueller, and Kelllleth F. Reardon

Modeling Root Uptake and Transport of Trichloroethylene - Jiang Hu, Muralidharan Narayanan, L.C. Davis, and L. E. Erickson

An Experimental Study of Trichloroethylene Fluxes into the Atmosphere - Qizhi Zhang, Lawrence C. Davis, and Larry E. Erickson

Toluene Contamination Enhances Populations of Toluene Degraders in the Rhizosphere - Xiaowei Wu and L.C. Davis

Microbial Transport in a Pilot-Scale Biological Treatment Zone - Daniel J. Adams, M.A. Malusis, K. F. Reardon, C. D. Shackelford, AI W. Bourquin, and Douglas C. Mosteller

A Model of the Effects of Acclimation on the Cardiorespiratory and Thermoregulatory Systems - D. Downey and R. C. Seagrave

The Effects of Physiological Intermittent Pressures on Cartilage Regeneration - Scott E. Carver and Carole A. Heath

Design of a Bioartificial Nerve Graft for Regeneration in the Periphery - Gregory E. Rutkowski and Carole A. Heath

Intracellular Calcium Changes in Endothelial Cells Exposed to Flow and Agonist Stimulation - L. Worthen and M. Nollert

HL-60 Cell-Cell Interactions under Flow Mediated by Platelet Microparticles - S. B. Forlow and M. U. Nollert

Publication Date:

9-13-1997

Publisher:

Colorado State University

City:

Fort Collins, CO

Disciplines:

Biochemical and Biomolecular Engineering

Proceedings of the Twenty-Seventh Annual Biochemical Engineering Symposium

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