Filtered stillage from the distillation of ethanol made by yeast fermentation of hydrolyzed corn starch was subjected to gas chromatography-mass spectroscopy and to high-performance liquid chromatography. Nearly all the major chromatographic peaks were identified and quantified. Low molecular weight organics in the soluble part of corn stillage were lactic acid, glycerol, and alanine, as well as smaller amounts of ethanol, and various nonnitrogenous and nitrogenous acids, polyhydroxy alcohols, sugars, and glucosides.