Methods of extracting chitin and chitosan from fungal biomass using a solution of one or more ammonia compounds, amines, and/or alkaline silicate compounds. The solution dissolves and extracts amino acids, fatty acids and other carbohydrates from the fungal cells leaving chitin and/or chitosan, and the extractant may be recovered from the liquid by simple phase changes such as heating or cooling, dissociation into volatile components, distillation and/or solidification and separation of immiscible extractants. Further lipid removal may be achieved with one or more organic solvents, which may also be recovered by distillation.
US 9,249,235 B2
Johannes van Leeuwen; Daniel Rudnick
van Leeuwen, Johannes; Norton, Glenn A.; Ndlela, Sipho C.; and Rudnick, Daniel, "Processes for isolating chitin and chitosan from fungal biomass" (2016). Civil, Construction and Environmental Engineering Patents. 2.