Fluidized bed reactors are important assets of many industrial applications because they provide uniform temperature distributions, low pressure drops, and high heat/mass rates. Characterizing the hydrodynamics of a fluidized bed is important to better understand the behavior of these multiphase flow systems. The hydrodynamic behavior in a cold flow 3D fluidized bed, with and without acoustic intervention, using X-ray computed tomography is investigated in this study. Experiments are carried out in a 10.2 cm ID fluidized bed filled with glass beads, with material density of 2600 kg/m³ and particle size ranges between 212–600 μm. In this study, three different bed height-to-diameter ratios are examined: H/D = 0.5, 1 and 1.5. Moreover, the sound frequency of the loudspeaker used as the acoustic source is fixed at 150 Hz with a sound pressure level of 120 dB. Local time-average gas holdup results show that the fluidized bed under the presence of an acoustic field provides a more uniform fluidization, the bed exhibits less channeling, and the jetting phenomena produced by the distributor plate is less prominent when compared to no acoustic field. Thus, acoustic intervention affects the hydrodynamic behavior of the fluidized bed.