Study I examined the effects of sprint training on muscle glycolysis in greyhounds. Training consisted of either a low volume regimen (LV) of 12 400-meter sprints during a six-week period, or a high volume regimen (HV) totalling 30 sprints. Before and after training, responses to 400-meter trial sprints were used to determine muscle glycogen utilization, lactate accumulation, and muscle buffer capacity;Sprint times were not improved following training. Training increased (p < 0.05) post-sprint blood lactate accumulation in both LV (15.0 ± 1.4 vs 17.6 ± 1.1 mmol· l[superscript]-1) and HV (14.0 ± 1.4 vs 17.3 ± 1.4 mmol· l[superscript]-1), whereas muscle lactate accumulation and pH were similar. Glycogen utilization increased (p < 0.05) following HV training (16.3 ± 2.8 vs 25.7 ± 3.0 mmoles glucose·kg wet wt[superscript]-1), but remained unchanged in LV. Maximal phosphofructokinase (PFK) activity was increased (p < 0.05) in HV (43.3 ± 1.3 vs 47.4 ± 1.4 mmol·kg body wt[superscript]-1·min[superscript]-1);Muscle buffer capacity was not affected by LV training, whereas a trend (p = 0.09) towards an increased buffer capacity occurred in HV (58.4 ± 4.6 vs 66.7 ± 5.8 Slykes). Increased glycogen utilization and PFK activity in HV suggests an enhanced glycolytic capacity with the higher training volume;Study II examined the effects of NaHCO[subscript]3 ingestion (300 mg·kg body wt[superscript]-1) on lactate accumulation, acid-base balance, and performance in greyhounds sprinting over 603.5 meters. Ten greyhounds performed a NaHCO[subscript]3 and control trial in a crossover design. Arterial blood was analyzed for lactate, pH, HCO[subscript]3[superscript]- (bicarbonate), and base excess (BE). Muscle biopsies were analyzed for lactate and pH;Two minute post-exercise blood lactate accumulation was increased (p < 0.05) after NaHCO[subscript]3 ingestion (20.4 ± 1.6 mmol· l[superscript]-1) when compared to control (16.9 ± 1.3 mmol· l[superscript]-1), whereas post-exercise muscle lactate accumulation was similar. Post-exercise muscle hydrogen ion (H[superscript]+) accumulation was higher (p < 0.05) after NaHCO[subscript]3 ingestion (158.8 ± 8.8 nmol· l[superscript]-1) when compared to control (137.0 ± 5.3 nmol· l[superscript]-1). Muscle H[superscript]+ recovery half-time (7.2 ± 1.6 min) and time to reach pre-dose values (22.2 ± 2.4 min) were reduced (p < 0.05) in the NaHCO[subscript]3 trial when compared to control (11.3 ± 1.6 and 32.9 ± 4.0 min). No trial differences were found for blood H[superscript]+ recovery curves, lactate recovery curves, performance times or muscle buffer capacities;In this study, NaHCO[subscript]3 ingestion increased blood lactate accumulation, but did not reduce the magnitude of the muscle or blood acid-base disturbance associated with a 603.5 m sprint task.