Abbreviated identification scheme for Eschericia coli in swine feces
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
Antimicrobial susceptibility profiles of Escherichia coli (EC) are often used to monitor the effect of antimicrobial use regimens on the antimicrobial resistance (AR) reservoir in animal species. Epidemiological studies of AR may involve the identification of thousands of bacterial isolates, so complete biochemical identification of EC can be prohibitively expensive and time consuming. In this study an abbreviated biochemical scheme using colony phenotype and the indole test results in a sensitivity and specificity of 91.7% and 100% respectively for identification of EC as compared to a commercial biochemical identification kit. This abbreviated scheme results in over US$500 savings per 100 candidate EC isolates identified. These savings have significant benefits to the economics of conducting epidemiologic investigations of AR.