The effect of gamma-rays on variability for three quantitative characters (number of pods, seeds, and grain yield per plant) of soybean were studied. In the M2 generation, the shifts of mean values of irradiated material for all the polygenic characters occurred towards the positive or negative direction. However, the coefficient of variability increased in all the treatments for the attributes studied. This additional genetic variability would provide an opportunity for improvement of soybean by selection.