Dry matter intake, milk production, and milk production efficiency were evaluated in 84 Holstein dairy cows fed either a control diet or a control diet plus the direct-fed microbial Bovamine. Neither milk nor ECM production were affected by feeding Bovamine. Feeding Bovamine, however, decreased DMI by 3.59%, resulting in an improvement in milk production efficiency and ECM production efficiency of 6.1% and 5.3%, respectively. Inclusion of Bovamine in dairy cattle diets should be considered to increase milk production efficiency.