Identification ofspecific geneor quantitative trait locus that responds to specific stimulus is an important goal for genetic improvementprograms. In a heat stress experiment with 3 distinct chicken lines, we found Angiopoietin-like 4 (ANGPTL4) to be differentially expressed in all 3 lines during acute and chronic heat stress.This outcome suggests the need to further exploreANGPTL4 as a potential candidate gene for selection to improve chicken response to heat stress.