Tillage and crop rotation systems have significant long-term effects on soil productivity and soil quality components such as soil carbon and other soil physical, biological, and chemical properties. Additionally, tillage and crop rotations have impacts on weed and soil disease control. There is need for a well-defined, long-term tillage and crop rotation studies across the different soils and climate conditions in the state. The objective of this study was to evaluate the long-term effects of different tillage systems and crop rotations on soil quality and on corn and soybean yields.