The soybean aphid, Aphis glycines (Hemiptera: Aphididae), is a consistent pest of soybean in Iowa. Current management is heavily reliant on the use of insecticides, which can be expensive and time consuming to apply. Soybean varieties resistant to the aphid are available. These varieties primarily include one resistance gene (Rag1) for soybean aphid control. Varieties incorporating two genes (Rag1 + Rag2) have recently become available. We sought to compare soybean lines susceptible to the soybean aphid with lines carrying a single resistance gene (Rag1 or Rag2) and a line carrying two resistance genes (Rag1 + Rag2). We evaluated the lines based on aphid control and yield protection.