Document Type

Article

Publication Version

Published Version

Publication Date

7-2015

Journal or Book Title

Applications in Plant Sciences

Volume

3

Issue

7

First Page

1

Last Page

5

DOI

10.3732/apps.1500028

Abstract

Premise of the study: Single-nucleotide polymorphism (SNP) primers were developed for a native North American desert fig, Ficus petiolaris (Moraceae), to provide markers for population genetic studies designed to quantify patterns of gene flow across a complex landscape. Methods and Results: Transcriptome sequencing and bioinformatic protocols were implemented to discover SNPs in single-copy protein-coding genes. Multiplexes of 30 nuclear and 24 organellar (chloroplast and mitochondrial) SNPs were selected for primer development and genotyping on the Sequenom MASSArray System. Of these 54 loci, 49 reliably amplified across a panel of 96 F. petiolaris individuals. Conclusions: This study has provided SNP primers that can be applied in future studies investigating population genetics of F. petiolaris and its coevolution with associated pollinating and nonpollinating fig wasps.

Comments

This article is from Applications in Plant Sciences 3 (2015): 1500028, doi:10.3732/apps.1500028. Posted with permission.

Rights

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Copyright Owner

Nicholas G. Davis, Derek D. Houston, and John D. Nason

Language

en

File Format

application/pdf

Share

COinS