Document Type

Article

Publication Date

10-22-2010

Journal or Book Title

Vector-Borne and Zoonotic Diseases

Volume

10

Issue

8

First Page

777

Last Page

783

DOI

10.1089/vbz.2009.0196

Abstract

A total of 191,244 mosquitoes from 24 species were collected in the Yucatan Peninsula of Mexico from January to December 2008, and tested for the presence of cytopathic virus by virus isolation in Vero cells. Eighteen virus isolates were obtained, all of which were orthobunyaviruses. These were identified by reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing as Cache Valley virus (n = 17) and South River virus (n = 1). A subset (n = 20,124) of Culex quinquefasciatus collected throughout the year was further tested by RT-PCR using flavivirus-specific primers. Flavivirus RNA was present in this mosquito species year-round. The overall flavivirus minimal infection rate, expressed as the number of positive mosquito pools per 1000 mosquitoes tested, was 7.7 and the monthly flavivirus minimal infection rates ranged from 4.3 to 16.6. Approximately one-third of the RT-PCR products were sequenced and all corresponded to Culex flavivirus, a recently discovered insect-specific flavivirus.

Comments

This is a copy of an article published in the Vector-Borne and Zoonotic Diseases ©2010 Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at: http://online.liebertpub.com.

Copyright Owner

Mary Ann Liebert, Inc.

Language

en

File Format

application/pdf