Document Type

Article

Publication Date

1999

Journal or Book Title

Insect Molecular Biology

Volume

8

Issue

2

First Page

213

Last Page

221

DOI

10.1046/j.1365-2583.1999.820213.x

Abstract

The genetic variability of seven European corn borer populations, Ostrinia nubilalis, from North America and Europe was assessed by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing. The nuclear ribosomal internal transcribed spacer 1 (ITS-1) region (≈ 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The smartweed borer, Ostrinia obumbratalis, and south-Western corn borer, Diatraea grandiosella, were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS-1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The lack of variation in the ITS-1 of European corn borer was confirmed by DNA sequence analysis. The genetic similarity of European corn borer populations, despite their wide geographical range and physiological differences, may be explained by a relatively recent origin for the voltinism and pheromone races, gene flow among races, and/or expansion from genetic bottlenecks.

Comments

This article is from Insect Molecular Biology; 8 (1999);213-221; doi:10.1046/j.1365-2583.1999.820213.x .

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Copyright Owner

Blackwell Science Ltd

Language

en

File Format

application/pdf

Included in

Entomology Commons

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