Document Type

Article

Publication Date

2005

Journal or Book Title

Journal of Insect Science

Volume

5

Issue

19

First Page

1

Last Page

12

DOI

10.1673/031.005.1901

Abstract

Ommochrome-binding proteins function in coloration and detoxification pathways by transporting tryptophan metabolites, and increase in hemolymph concentration prior to diapause. Two ommochrome-binding protein genes from the European corn borer Ostrinia nubilalis (Hübner) (Onobp1 and Onobp2; GenBank accession nos. AY819651 to AY819655 and AY862870) were isolated. Relatedness to OBP-encoding genes was suggested by peptide similarity, phylogenetic reconstruction, and expression data. 21 single nucleotide polymorphisms between obp1 and 23 polymorphisms between obp2 alleles were identified, and resultant genomic markers were inherited in a Mendelian fashion. RT-PCR showed fat body specific Onobp1 and Onobp2 transcription. The Onobp1 transcript was RT-PCR amplified from fat body of 5th instars, whereas Onobp2 was expressed in fat body of 4th and 5th instars, and peaked in 5th instar wandering and 1 week old diapausing larvae. Expression suggests gene duplicates are maintained by change in temporal expression. The significance of Onobp1 and 2 gene products to O. nubilalis diapause physiology requires additional investigation.

Comments

This article is from Journal of Insect Science; 5 (2005); 1-12; doi: 10.1673/031.005.1901

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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