Tillage system and crop rotation have significant long-term effects on soil productivity and soil components such as soil carbon as well as on soil physical, biological, and chemical properties. In addition, both tillage and crop rotation have effects on weed and soil disease control. There is a definite need for well-defined, long-term tillage and crop rotation studies across the different soils and climate conditions in Iowa. The objective of this study was to evaluate the long-term effects of different tillage systems and crop rotations on soil productivity.