The continental climate in Iowa is important when selecting grape cultivars that are tolerant of low-temperature stresses. Many coldregulated (COR) proteins respond to freezing stress, and are able to protect cellular membranes from cold-induced dehydration damage. It is unknown which proteins are important for cultivars to withstand extreme temperature fluctuations and low temperatures that can injure over-wintering canes. The objective of this investigation is to observe the ability of two grape cultivars to survive extreme temperature fluctuations during coldacclimation, mid winter, and deacclimation. Experiments will be conducted to determine and compare changes in proteins during cold acclimation and deacclimation in canes for Frontenac and Seyval blanc grape cultivars.