Document Type

Article

Publication Date

10-4-2006

Journal or Book Title

Journal of Agricultural and Food Chemistry

Volume

54

Issue

20

First Page

7422

Last Page

7428

DOI

10.1021/jf061624c

Abstract

Efficient separation and accurate quantification of sphingolipids (SL) are important for studying SL concentrations and biological functions. The objectives of this study were to develop effective methods for the separation and quantification of SL and to determine the relationship between palmitate and SL contents of mature soybean seeds. Methods using column chromatography and high-performance liquid chromatography−evaporative light scattering detector (HPLC-ELSD) were developed to separate and quantify glucosylceramide (GlcCer) and ceramide (Cer) in 15 soybeans lines in which palmitate content ranged from 3.7 to 40.7%. There were significant differences among the lines for GlcCer (83.4−397.6 nmol/g) and major Cer contents (8.4−20.7 nmol/g) on a dry weight basis. The correlations of palmitate content with GlcCer and Cer concentrations were not significant. The results indicated that the palmitate content of soybean seed did not affect their GlcCer and Cer contents. Genetic factors other than those that control palmitate content seemed to be responsible for the variation among soybean lines for GlcCer and Cer contents.

Comments

Posted with permission from Journal of Agricultural and Food Chemistry, 54, no. 20 (2006): 7422–7428, doi: 10.1021/jf061624c. Copyright 2006 American Chemical Society.

Copyright Owner

2006

Language

en

File Format

application/pdf

Share

COinS