Document Type

Article

Publication Date

1-11-2012

Journal or Book Title

Journal of Agricultural and Food Chemistry

Volume

60

Issue

1

First Page

451

Last Page

457

DOI

10.1021/jf203629w

Abstract

An effective process for the enzymatic synthesis of oleoylethanolamide is described in this study. The process included purification of a commercial oleic acid product and then optimization of the reaction between the purified oleic acid and ethanolamine in the presence of hexane and a lipase. Under the optimal amidation reaction conditions identified, oleoylethanolamide was obtained with 96.6% purity. The synthesis was also conducted on a large scale (50 mmol of each of the reactants), and oleoylethanolamide purity and yield after crystallization purification were 96.1 and 73.5%, respectively. Compared to the previous studies, the current method of preparing high-purity oleoylethanolamide is more effective and economically feasible. The scalability and ease for such synthesis make it possible to study the biological and nutritional functions of the cannabinoid-like oleoylethanolamide in animal or human subjects.

Comments

Posted with permission from Journal of Agricultural and Food Chemistry,60, no. 1 (2012): 451–457, doi: 10.1021/jf203629w. Copyright 2011 American Chemical Society.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

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