Journal or Book Title
Journal of Agricultural and Food Chemistry
In this paper we characterize the metabolic fingerprint and first reported anti-inflammatory activity of Hypericum gentianoides. H. gentianoides has a history of medical use by Native Americans, but it has been studied very little for biological activity. High-performance liquid chromatography (HPLC) and liquid chromatography−electrospray ionization−mass spectrometry (LC-ESI-MS) analyses of a methanol extract show that H. gentianoides contains a family of over nine related compounds that have retention times, mass spectra, and a distinctive UV absorption spectra characteristic of certain acyl-phloroglucinols. These metabolites are abundant relative to other secondary products present in H. gentianoides, accounting for approximately 0.2 g per gram of dry plant tissue. H. gentianoides methanol extracts and a specific semipreparative HPLC fraction from these extracts containing the putative acyl-phloroglucinols reduce prostaglandin E2 synthesis in mammalian macrophages.
American Chemical Society
Hillwig, Matthew L.; Hammer, Kimberly D.P.; Birt, Diane F.; and Wurtele, Eve S., "Characterizing the Metabolic Fingerprint and Anti-inflammatory Activity of Hypericum gentianoides" (2008). Food Science and Human Nutrition Publications. 19.