Journal or Book Title
Journal of Agricultural and Food Chemistry
Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. To calibrate the FTIR-PAS analysis, samples from each diet were analyzed using an enzymatic assay. A partial least-squares cross-validation plot generated from the enzymatic assay and FTIR-PAS spectral results for starch fit the ideal curve with a R(2) of 0.997. A principal component analysis plot of components 1 and 2 showed that spectra from diets clustered significantly from each other. This study clearly showed that FTIR-PAS can accurately quantify starch content and identify the form of starch in complex matrices.
Reprinted with permission from Journal of Agricultural and Food Chemistry, 61(8):1818-1822. doi: 10.1021/jf3042616 . Copyright (2013) American Chemical Society.
American Chemical Society
Anderson, Timothy J.; Ai, Yongfeng; Jones, Roger W.; Houk, Robert S.; Jane, Jay-lin; Zhao, Yinsheng; Birt, Diane F.; and McClelland, John F., "Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy" (2013). Food Science and Human Nutrition Publications. 79.