Campus Units

Food Science and Human Nutrition, Genetics, Development and Cell Biology

Document Type

Article

Publication Version

Published Version

Publication Date

11-2-2015

Journal or Book Title

Journal of Agricultural and Food Chemistry

Volume

63

Issue

46

First Page

10170

Last Page

10180

DOI

10.1021/acs.jafc.5b04109

Abstract

A study of yolks stored up to 168 d at −20 °C was conducted to determine the gelation behavior and mechanism of freeze–thawed yolk. Methods used were rheology, native and sodium dodecyl sulfate polyacrylamide gel electrophoresis (native- and SDS-PAGE), differential scanning calorimetry (DSC), transmission electron microscopy (TEM), particle size analysis, and proton nuclear magnetic resonance (1H NMR) spectroscopy for matrix mobility. Results indicate that both constituents of plasma and granules contributed to gelation of yolk under freezing. PAGE analyses suggest that granular proteins participated in aggregation during freeze–thaw. Increasing gel strength and particle size and decreasing water and lipid–water mobility indicate that lipoproteins or apolipoproteins aggregated. At storage times ≥84 d, increased protein and lipid mobility, the detection of smaller particles, and secondarily increased gel strength suggest the liberation of protein or lipoprotein components from previously formed aggregates and further aggregation of these constituents. Disruption of the gelled yolk matrix observed with TEM supported that ice crystal formation was required for gelation to occur. A two-stage dynamic gelation model is thus proposed.

Comments

Reprinted (adapted) with permission from Journal of Agricultural and Food Chemistry, 63(46); 10170-10180. Doi: 10.1021/acs.jafc.5b04109. Copyright 2015 American Chemical Society.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

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