Campus Units

Genetics, Development and Cell Biology, Bioinformatics and Computational Biology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2011

Journal or Book Title

Nature Methods

Volume

8

Issue

1

First Page

67

Last Page

69

DOI

10.1038/nmeth.1542

Abstract

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.

Comments

This is a manuscript of an article from Nature Methods 8 (2011): 67, doi: 10.1038/nmeth.1542. Posted with permission.

Copyright Owner

Nature American, Inc.

Language

en

File Format

application/pdf

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