Campus Units

Genetics, Development and Cell Biology, Bioinformatics and Computational Biology

Document Type

Article

Publication Version

Published Version

Publication Date

6-7-2016

Journal or Book Title

Scientific Reports

Volume

6

First Page

27413

DOI

10.1038/srep27413

Abstract

We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core, and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force-generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame thereby providing a mechanism for molecular detection and interrogation at the ring-frame interface. As proof of principle we use this system to evaluate base stacking adhesion and demonstrate detection of a soluble nucleic acid viral genome mimic.

Comments

This article is from Scientific Reports 6 (2016): 27413, doi: 10.1038/srep27413.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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