Campus Units

Genetics, Development and Cell Biology, Agronomy

Document Type

Article

Publication Version

Published Version

Publication Date

2009

Journal or Book Title

Genes & Development

Volume

23

First Page

755

Last Page

765

DOI

10.1101/gad.1776909

Abstract

Barbara McClintock reported that the Ac/Ds transposable element system can generate major chromosomal rearrangements (MCRs), but the underlying mechanism has not been determined. Here, we identified a series of chromosome rearrangements derived from maize lines containing pairs of closely linked Ac transposable element termini. Molecular and cytogenetic analyses showed that the MCRs in these lines comprised 17 reciprocal translocations and two large inversions. The breakpoints of all 19 MCRs are delineated by Ac termini and characteristic 8-base-pair target site duplications, indicating that the MCRs were generated by precise transposition reactions involving the Ac termini of two closely linked elements. This alternative transposition mechanism may have contributed to chromosome evolution and may also occur during V(D)J recombination resulting in oncogenic translocations.

Comments

This article is from Genes & Development 23 (2009): 755, doi: 10.1101/gad.1776909. Posted with permission.

Copyright Owner

Cold Spring Harbor Laboratory Press

Language

en

File Format

application/pdf