Campus Units

Biochemistry, Biophysics and Molecular Biology, Genetics, Development and Cell Biology, Veterinary Clinical Sciences, Neuroscience

Document Type

Article

Publication Version

Published Version

Publication Date

2008

Journal or Book Title

Archives of Ophthalmology

Volume

126

Issue

11

First Page

1530

Last Page

1536

DOI

10.1001/archopht.126.11.1530

Abstract

Objective To determine the distribution of 2 intracellular oxygen-carrying molecules, neuroglobin (NGB) and cytoglobin (CYGB), in specific retinal cell types of human retinas.

Methods Specific antibodies against NGB and CYGB were used in immunohistochemical studies to examine their distribution patterns in human retinal sections. Double-labeling studies were performed with the anti-NGB and anti-CYGB antibodies along with antibodies against neuronal (microtubule-associated protein 2, class III β-tubulin [TUJ1], protein kinase C alpha, calretinin) and glial (vimentin, glial fibrillary acid protein) markers. Confocal microscopy was used to examine the retinal sections.

Results Immunohistochemical analysis of human retinal tissue showed NGB and CYGB immunoreactivity in the ganglion cell layer, inner nuclear layer, inner and outer plexiform layers, and retinal pigment epithelium. Neuroglobin immunoreactivity was also present in the outer nuclear layer and photoreceptor inner segments. Neuroglobin and CYGB were coexpressed in the neurons in the ganglion cell layer and inner nuclear layer but not within glial cells.

Conclusion Neuroglobin and CYGB are colocalized within human retinal neurons and retinal pigment epithelium but not within glial cells.

Clinical Relevance Our results suggest that NGB and CYGB may serve a neuroprotective role as scavengers of reactive oxygen species and therefore should be considered when developing therapeutic strategies for treatment of hypoxia-related ocular diseases.

Comments

This article is from Archives of Ophthalmology 126 (2008): 1530, doi: 10.1001/archopht.126.11.1530.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf