Campus Units

Genetics, Development and Cell Biology, Biomedical Sciences, Neuroscience

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

7-2014

Journal or Book Title

Biotechnology Journal

Volume

9

Issue

7

First Page

921

Last Page

933

DOI

10.1002/biot.201400019

Abstract

Adult hippocampal progenitor cells (AHPCs) are generally maintained as a dispersed monolayer population of multipotent neural progenitors. To better understand cell-cell interactions among neural progenitors and their influences on cellular characteristics, we generated free-floating cellular aggregates, or neurospheres, from the adherent monolayer population of AHPCs. Results from in vitro analyses demonstrated that both populations of AHPCs were highly proliferative under maintenance conditions, but AHPCs formed in neurospheres favored differentiation along a glial lineage and displayed greater migrational activity, than the traditionally cultured AHPCs. To study the plasticity of AHPCs from both populations in vivo, we transplanted GFP-expressing AHPCs via intraocular injection into the developing rat eyes. Both AHPC populations were capable of surviving and integrating into the developing host central nervous system, but considerably more GFP-positive cells were observed in the retinas transplanted with neurosphere AHPCs, compared to adherent AHPCs. These results suggest that the culture configuration during maintenance for neural progenitor cells (NPCs) influences cell fate and motility in vitro as well as in vivo. Our findings have implication for understanding different cellular characteristics of NPCs according to distinct intercellular architectures and for developing cell-based therapeutic strategies using lineage-committed NPCs.

Comments

This is the peer reviewed version of the following article: Oh, J., Daniels, G. J., Chiou, L. S., Ye, E.-A., Jeong, Y.-S. and Sakaguchi, D. S. (2014), Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages. Biotechnology Journal, 9: 921–933, which has been published in final form at doi:10.1002/biot.201400019. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

Copyright Owner

Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Language

en

File Format

application/pdf

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