Campus Units

Genetics, Development and Cell Biology, Veterinary Clinical Sciences, Neuroscience

Document Type

Article

Publication Version

Published Version

Publication Date

8-2010

Journal or Book Title

Graefe's Archive for Clinical and Experimental Ophthalmology

Volume

248

Issue

8

First Page

1105

Last Page

1116

DOI

10.1007/s00417-010-1336-7

Abstract

Background

To characterize the molecular and functional status of the rat retina and optic nerve after acute elevation of intraocular pressure (IOP).

Methods

Retinal ischemia was induced in rats by increasing the IOP (110 mmHg/60 minutes). Microarray analysis, quantitative RT-PCR (qRT-PCR) and immunohistochemistry were used to characterize retinal tissue. PLGA microspheres containing neurotrophic factors (BDNF, GDNF, or CNTF) or empty microspheres were injected into the vitreous of operated animals 1 day after elevation of IOP. Pupil light reflex (PLR) parameters and electroretinograms (ERG) were monitored at multiple time points during the 60-day postoperative recovery period.

Results

Molecular analysis showed a significant intrinsic up-regulation of CNTF at 10 and 25 days after induction of the acute ocular hypertension (p = 0.0067). Molecular tissue analysis of GDNF and its receptors (GDNFR1, GDNFR2), and BDNF and its receptor (trkB) showed no change in expression. Animals that received CNTF microspheres had no significant functional recovery compared to animals which received blank microspheres (p > 0.05). Animals that received GDNF or BDNF microspheres showed significant PLR recovery (p < 0.05 and p < 0.001 respectively) compared to non-treated animals.

Conclusions

Continuous release of neurotrophic growth factors (NGFs) significantly protects optic nerve function in the experimental model of retinal ischemia observed by PLR analysis.

Comments

This article is from Graefe's Archive for Clinical and Experimental Ophthalmology 248 (2010): 1105, doi: 10.1007/s00417-010-1336-7.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf