Campus Units

Genetics, Development and Cell Biology, Agronomy

Document Type

Article

Publication Version

Published Version

Publication Date

2-2015

Journal or Book Title

Plant Biotechnology Journal

Volume

13

Issue

7

First Page

1002

Last Page

1010

DOI

10.1111/pbi.12344

Abstract

Transcription activator-like effector nuclease (TALEN) technology has been utilized widely for targeted gene mutagenesis, especially for gene inactivation, in many organisms, including agriculturally important plants such as rice, wheat, tomato and barley. This report describes application of this technology to generate heritable genome modifications in maize. TALENs were employed to generate stable, heritable mutations at the maize g lossy2 ( gl2 ) locus. Transgenic lines containing mono- or di-allelic mutations were obtained from the maize genotype Hi-II at a frequency of about 10% (nine mutated events in 91 transgenic events). In addition, three of the novel alleles were tested for function in progeny seedlings, where they were able to confer the glossy phenotype. In a majority of the events, the integrated TALEN T-DNA segregated independently from the new loss of function alleles, producing mutated null-segregant progeny in T1 generation. Our results demonstrate that TALENs are an effective tool for genome mutagenesis in maize, empowering the discovery of gene function and the development of trait improvement.

Comments

This article is published as Char, Si Nian, Erica Unger‐Wallace, Bronwyn Frame, Sarah A. Briggs, Marcy Main, Martin H. Spalding, Erik Vollbrecht, Kan Wang, and Bing Yang. "Heritable site‐specific mutagenesis using TALENs in maize." Plant biotechnology journal 13, no. 7 (2015): 1002-1010. 10.1111/pbi.12344

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Society for Experimental Biology

Language

en

File Format

application/pdf

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