Campus Units

Genetics, Development and Cell Biology, Animal Science, Neuroscience, Food Science and Human Nutrition, Kinesiology, Microbiology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2007

Journal or Book Title

Food and Agricultural Immunology

Volume

18

Issue

3-4

First Page

221

Last Page

236

DOI

10.1080/09540100701797363

Abstract

It has been suggested that Echinacea has anti-inflammatory activity in vivo. Nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-1beta are important mediators in the inflammatory response. The effect of alcohol extracts of E. angustifolia (EA), E. pallida (EPA) and E. purpurea (EP) on the production of these inflammatory mediators in both LPS-stimulated RAW 264.7 macrophages in vitro and murine peritoneal exudate cells (PECs) in vivo were investigated. As macrophages produce these inflammatory mediators in response to pathogenic infection, parallel cultures of macrophages were studied for phagocytosis and intracellular killing of Salmonella enterica. EPA and EP in vitro inhibited NO production and TNF-α release in a dose-dependent manner. RAW 264.7 cells treated with EA or EP showed decreased killing over 24 h, although EA enhanced bacterial phagocytosis. Upon bacterial infection, RAW 264.7 cells produce high levels of NO; however, an Echinacea-mediated decrease in NO production was observed. Echinacea alcohol extracts administered orally at 130 mg/kg per day for seven days had a weak effect on NO production and phagocytosis by LPS-stimulated PECs. The results indicated that all Echinacea species significantly decreased inflammatory mediators in vitro, however, only EA and EP reduced bacterial killing. Oral administration of Echinacea alcohol extracts did not adversely affect the development and anti-bacterial function of inflammatory PECs in vivo, however, NO production was decreased during bacterial infection of PECs.

Comments

This is an Accepted Manuscript of an article published by Taylor & Francis in Food and Agricultural Immunology on 2007, available online: http://www.tandfonline.com/10.1080/09540100701797363

Copyright Owner

Taylor & Francis

Language

en

File Format

application/pdf

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