Campus Units

Genetics, Development and Cell Biology, Animal Science, Kinesiology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2009

Journal or Book Title

Cytokine

Volume

46

Issue

2

First Page

267

Last Page

272

DOI

10.1016/j.cyto.2009.02.007

Abstract

Background—Members of the genus Echinacea are used medicinally to treat upper respiratory infections such as colds and influenza. The aim of the present investigation was to characterize the phytomedicinal properties of the American federally endangered species Echinacea tennesseensis.

Methods—Fifty-percent ethanol tinctures were prepared from roots, stems, leaves, and flowers and tested separately for their ability to influence production of IL-1β, IL-2, IL-10, and TNF-α as well as proliferation by young human adult peripheral blood mononuclear cells (PMBC) in vitro. Tincture aliquots were stored at three different temperatures (4°, −20°C, and −80°C) for 21 h before testing. At one-month post-extraction, tinctures stored at −20°C were tested again for cytokine modulation. Phytochemical analyses were performed using HPLC.

Results—Fresh root, leaf, and flower tinctures stimulated PBMC proliferation. Fresh root tinctures alone stimulated IL-1β, IL-10, and TNF-α production. No tinctures modulated IL-2 production. Stem tinctures showed no activity. Storage temperature did not influence any outcomes. Root tinctures maintained their ability to modulate IL-1β, IL-10, and TNF-α production after one month of storage at −20°C.

Conclusions—These results suggest E. tennesseensis harbors phytomedicinal properties that vary by plant organ, with roots demonstrating the strongest activities.

Comments

This is a manuscript of an article from Cytokine 46 (2009): 267, doi: 10.1016/j.cyto.2009.02.007. Posted with permission.

Rights

© 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Owner

Elsevier Ltd

Language

en

File Format

application/pdf

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