Campus Units

Genetics, Development and Cell Biology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2013

Journal or Book Title

Developmental Cell

Volume

26

Issue

1

First Page

59

Last Page

72

DOI

10.1016/j.devcel.2013.06.005

Abstract

Cone photoreceptors carry out phototransduction in daylight conditions and provide the critical first step in color vision. Despite their importance, little is known about the developmental mechanisms involved in their generation, particularly how they are determined relative to rod photoreceptors, the cells that initiate vision in dim light. Here, we report the identification of a cisregulatory module (CRM) for the thyroid hormone receptor beta (Thrb) gene, an early cone marker. We found that ThrbCRM1 is active in progenitor cells biased to the production of cones and an interneuronal cell type, the horizontal cell (HC). Molecular analysis of ThrbCRM1 revealed that it is combinatorially regulated by the Otx2 and Onecut1 transcription factors. Onecut1 is sufficient to induce cells with the earliest markers of cones and HCs. Conversely, interference with Onecut1 transcriptional activity leads to precocious rod development, suggesting that Onecut1 is critically important in defining cone versus rod fates.

Comments

This is a manuscript of an article from Developmental Cell 26 (2013): 59, doi: 10.1016/j.devcel.2013.06.005. Posted with permission.

Copyright Owner

Elsevier Inc.

Language

en

File Format

application/pdf

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