Iowa State University Veterinarian Records, RS 22/6/0/9, University Archives, Special Collections Department, Iowa State University, http://www.add.lib.iastate.edu/spcl/arch/rgrp/22-6-0-9.html
The infectious agent of sporadic bovine encephalomyelitis has now been propagated for nearly three years in developing chick embryos.This has afforded a convenient method of propagation while studying the causal agent. It is interesting that during all of this time in chick embryos, which represents more than 135 passages in series, there has been no apparent variation in the character of the infectious agent. It is as pathogenic for guinea pigs and cattle as when first isolated. Failure to recognize anything in the tissues of affected animals that would indicate the exact nature of the entity which causes the disease, led to an extension of the histological study to infected chick embryos, especially their membranes and yolk sacs.
McNutt, S. H. and Stearns, T. W.
"Sporadic Bovine Encephalomyelitis,"
Iowa State University Veterinarian: Vol. 5
, Article 8.
Available at: http://lib.dr.iastate.edu/iowastate_veterinarian/vol5/iss2/8