Document Type

Article

Publication Date

6-2008

Journal or Book Title

Journal of AOAC International

Volume

91

Issue

4

First Page

884

Last Page

891

Abstract

AQUI-S is a fish anesthetic/sedative that is approved for use in a number of countries throughout the world and has the potential for use in the United States. The active ingredient in AQUI-S is isoeugenol. A method for determining isoeugenol concentrations in edible fillet tissue is needed for regulatory purposes, including surveillance and potential use in studies fulfilling human food safety data requirements if U.S. Food and Drug Administration approval is pursued. A method was developed and evaluated for determining isoeugenol concentrations in fillet tissue using relatively common procedures and equipment. The method produced accurate and precise results with fillet tissue from 10 freshwater fish species. The percentage of isoeugenol recovered from samples fortified with isoeugenol at nominal concentrations of 1, 50, and 100 g/g for all species was always >80 and <97. Within-day precision for samples fortified at those same concentrations was 10, and day-to-day precision was 4.0. Method precision with fillet tissue containing biologically incurred isoeugenol was 8.1. There were no or minimal chromatographic interferences in control fillet tissue extracts from 9 of the 10 species. The method detection limits for all but one species ranged from 0.004 to 0.014 g/g, and the quantitation limits ranged from 0.012 to 0.048 g/g.

Comments

This article is from Journal of AOAC International 91 (2008): 884–891.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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